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验证数据展示

  • IP experiment of HepG2 using 21326-1-AP

    IP experiment of HepG2 using 21326-1-AP

    IP result of anti-ZNF263 (IP:21326-1-AP, 4ug; Detection:21326-1-AP 1:300) with HepG2 cells lysate 4000ug.

  • IF Staining of U2OS using 21326-1-AP

    IF Staining of U2OS using 21326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF263 antibody (21326-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

ZNF263 Polyclonal antibody

ZNF263 Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 21326-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA and More (2)

别名

ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHepG2 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21326-1-AP targets ZNF263 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
文献引用应用WB, ChIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag15158

Product name: Recombinant human ZNF263 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 334-599 aa of BC008805

Sequence: LGRPHDRSQGDWAPPPEGGMEQALAGASSGRELGRPKELQPKKLHLCPLCGKNFSNNSNLIRHQRIHAAERLCMGVDCTEIFGGNPRFLSLHRAHLGEEAHKCLECGKCFSQNTHLTRHQRTHTGEKPYQCNICGKCFSCNSNLHRHQRTHTGEKPYKCPECGEIFAHSSNLLRHQRIHTGERPYKCPECGKSFSRSSHLVIHERTHERERLYPFSECGEAVSDSTPFLTNHGAHKAEKKLFECLTCGKSFRQGMHLTRHQRTHTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 263
别名 ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315
计算分子量 683 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC008805
基因名称 ZNF263
Gene ID (NCBI) 10127
RRIDAB_2878841
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14978
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF263 is a transcriptional repressor and activator that contains an N-terminal scanning domain and nine C2H2 zinc finger domains at the C-terminus, as well as a kruppel association box (KRAB) domain (PMID: 10567577). ZNF263 has been reported to be involved in early human embryonic development, cardiac regeneration, and adipogenesis (PMID: 37065635).

实验方案

Product Specific Protocols
IF protocol for ZNF263 antibody 21326-1-APDownload protocol
IP protocol for ZNF263 antibody 21326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Front Oncol

Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.

Authors - Min-Yun Cai
View Article
humanWB,ChIP

Pharmacogenomics J

The ZNF263/CPT1B axis regulates fatty acid β-oxidation to affect cisplatin resistance in lung adenocarcinoma

Authors - Renhe Yan
View Article