验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25502-1-AP targets ZNF215 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22026 Product name: Recombinant human ZNF215 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 110-304 aa of BC014538 Sequence: LQHPNNSKDMVTLIEDVIEMLEDEDMPCKDSALQMGSIKEKMKAGSRTGKPQEPVTFKDVVVEFSKEEWGQLDSAVKNLYRNVMLENFRNLNSLRKAHLLSKPFESLKLESKKKRWIMEKEIPRKTIFDMKSISGEESSHGVIMTRLTESGHPSSDAWKDGVSLLLPRLEYNDAVSAHCNLHLWVQVILLPQPPE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 215 |
| 别名 | BAZ2, BWSCR2-associated zinc finger protein 2, Zinc finger protein 215, ZKSCAN11 |
| 计算分子量 | 517 aa, 60 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC014538 |
| 基因名称 | ZNF215 |
| Gene ID (NCBI) | 7762 |
| RRID | AB_2880105 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL58 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF215 (Zinc Finger Protein 215) is a protein-coding gene that acts as a transcription factor, playing a significant role in embryonic development and various genetic disorders. ZNF215 contains SCAN, KRAB, and C2H2-type zinc finger domains. It functions in the nucleus by binding to specific DNA sequences to regulate the expression of downstream genes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNF215 antibody 25502-1-AP | Download protocol |
| WB protocol for ZNF215 antibody 25502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
