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验证数据展示

  • WB analysis using 26169-1-AP

    WB analysis using 26169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26169-1-AP (ZNF189 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26169-1-AP

    IF Staining of HeLa using 26169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF189 antibody (26169-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

ZNF189 Polyclonal antibody

ZNF189 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26169-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA

别名

Zinc finger protein 189

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, THP-1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26169-1-AP targets ZNF189 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 ZNF189 fusion protein Ag23264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 189
别名 Zinc finger protein 189
观测分子量68 kDa
GenBank蛋白编号BC126120
基因名称 ZNF189
Gene ID (NCBI) 7743
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75820
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF189 antibody 26169-1-APDownload protocol
IF protocol for ZNF189 antibody 26169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FEBS J

Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

Authors - Xiao Li
View Article