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验证数据展示

  • WB analysis of mouse kidney using 20385-1-AP

    WB analysis of mouse kidney using 20385-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20385-1-AP

    IHC staining of human heart using 20385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 10x lens).

ZNF146 Polyclonal antibody

ZNF146 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20385-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20385-1-AP targets ZNF146 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ZNF146 fusion protein Ag14223 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 146
别名 Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146
计算分子量 292 aa, 33 kDa
观测分子量 20-25 kDa, 33-35 kDa
GenBank蛋白编号BC005154
基因名称 ZNF146
Gene ID (NCBI) 7705
RRIDAB_10694154
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15072
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF146 antibody 20385-1-APDownload protocol
IHC protocol for ZNF146 antibody 20385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Reproduction

ZNF146 regulates cell cycle progression via TFDP1 and DEPDC1B in ovarian cancer cells

Authors - Ruixue Zhao
View Article
human,mouseWB,IHC

J Gastrointest Oncol

Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma.

Authors - Lei Bao
View Article