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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of SKOV-3 using 24531-1-AP

    WB analysis of SKOV-3 using 24531-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24531-1-AP

    IF Staining of HepG2 using 24531-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF140 antibody (24531-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ZNF140 Polyclonal antibody

ZNF140 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24531-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

pHZ 39, Zinc finger protein 140

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24531-1-AP targets ZNF140 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21379

Product name: Recombinant human ZNF140 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 79-162 aa of BC040561

Sequence: SESSGEIKDFSPKNVIYDDSSQYLIMERILSQGPVYSSFKGGWKCKDHTEMLQENQGCIRKVTVSHQEALAQHMNISTVERPYG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 140
别名 pHZ 39, Zinc finger protein 140
计算分子量 457 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC040561
基因名称 ZNF140
Gene ID (NCBI) 7699
RRIDAB_2879592
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52738
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF140 antibody 24531-1-APDownload protocol
IF protocol for ZNF140 antibody 24531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols