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验证数据展示

  • WB analysis of SH-SY5Y using 25216-1-AP

    WB analysis of SH-SY5Y using 25216-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 25216-1-AP

    IP experiment of SH-SY5Y using 25216-1-AP

    IP result of anti-ZNF124 (IP:25216-1-AP, 4ug; Detection:25216-1-AP 1:2000) with SH-SY5Y cells lysate 1600ug.

  • IHC staining of human gliomas using 25216-1-AP

    IHC staining of human gliomas using 25216-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25216-1-AP

    IF Staining of HepG2 using 25216-1-AP

    Immunofluorescent analysis of HepG2 cells using 25216-1-AP (ZNF124 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 25216-1-AP

    IF Staining of A431 using 25216-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF124 antibody (25216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ZNF124 Polyclonal antibody

ZNF124 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25216-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-937 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25216-1-AP targets ZNF124 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 ZNF124 fusion protein Ag17165 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 124
别名 HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124
计算分子量 351 aa, 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC099661
基因名称 ZNF124
Gene ID (NCBI) 7678
RRIDAB_2879965
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15973
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF124 antibody 25216-1-APDownload protocol
IHC protocol for ZNF124 antibody 25216-1-APDownload protocol
IF protocol for ZNF124 antibody 25216-1-APDownload protocol
IP protocol for ZNF124 antibody 25216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols