验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
25214-1-AP targets ZKSCAN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | ZKSCAN1 fusion protein Ag19721 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | zinc finger with KRAB and SCAN domains 1 |
别名 | 9130423L19Rik, KOX18, PHZ 37, Zinc finger protein 139, Zinc finger protein 36, Zinc finger protein KOX18, ZKSCAN1, ZNF139, ZNF36 |
计算分子量 | 563 aa, 64 kDa |
观测分子量 | 64 kDa |
GenBank蛋白编号 | BC022378 |
基因名称 | ZKSCAN1 |
Gene ID (NCBI) | 7586 |
RRID | AB_2879963 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P17029 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZKSCAN1(Zinc finger protein with KRAB and SCAN domains 1), also named as ZNF139, is a member in transcription factor zinc finger protein family(PMID: 24515389). Zinc finger protein 139 (ZNF139) gene is proved play an important role in gastric cancer, overexpression of ZNF139 correlated with tumor differentiation, invasion depth, clinical stage, lymphatic metastasis, and blood vessel invasion(PMID: 25436386, PMID: 25561801). The molecular weight of ZKSCAN1 is 64 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ZKSCAN1 antibody 25214-1-AP | Download protocol |
IF protocol for ZKSCAN1 antibody 25214-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |