验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
51021-1-AP targets Phospho-ZIPK (Ser311) in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | death-associated protein kinase 3 |
| 别名 | DAP kinase 3, DAP like kinase, DAPK3, Dlk, Phospho-ZIPK (Ser311), ZIP, ZIP kinase, ZIPK |
| 计算分子量 | 52.5 kDa |
| 观测分子量 | 52.5 kDa |
| GenBank蛋白编号 | BC126430 |
| 基因名称 | DAPK3 |
| Gene ID (NCBI) | 1613 |
| RRID | AB_614997 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43293 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAPK3(Death-associated protein kinase 3) is also named ZIPK(Zipper-interacting protein kinase), and Dlk (DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes(PMID:20038585). It has 2 isoforms produced by alternative splicing, named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IHC protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IP protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |