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ZIC4-Specific Polyclonal antibody

ZIC4-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 51003-1-AP

Print datasheet

Synonyms

Zic family member 4, ZIC4, Zinc finger protein ZIC 4

验证数据展示

  • IHC staining of mouse brain using 51003-1-AP

    IHC staining of mouse brain using 51003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 51003-1-AP

    WB analysis of human brain using 51003-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51003-1-AP targets ZIC4-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Zic family member 4
Synonyms Zic family member 4, ZIC4, Zinc finger protein ZIC 4
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberNM_032153
Gene Symbol ZIC4
Gene ID (NCBI) 84107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ZIC gene family includes 5 genes that are highly conserved across evolution. These genes encode zinc finger proteins that are expressed during development and maturation of the central nervous system and have critical roles in the development of the cerebellum. ZIC4, likes other member, may has roles in the nervous system development.

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Neural Tube Defects and ZIC4 Hypomethylation in Relation to Polycyclic Aromatic Hydrocarbon Exposure.

Authors - Yun Huang
View Article
humanWB,IHC

BMC Cancer

Expression of ZIC family genes in meningiomas and other brain tumors.

Authors - Aruga Jun J
View Article