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验证数据展示

  • WB analysis using 13118-2-AP (same clone as 13118-2-PBS)

    WB analysis using 13118-2-AP (same clone as 13118-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZFYVE16 Polyclonal antibody, PBS Only

ZFYVE16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13118-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13118-2-PBS targets ZFYVE16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3811

Product name: Recombinant human ZFYVE16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC032227

Sequence: MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTSALTCSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 16
别名
计算分子量 1539 aa, 169 kDa
观测分子量230-250 kDa
GenBank蛋白编号BC032227
基因名称 ZFYVE16
Gene ID (NCBI) 9765
RRIDAB_2288795
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3T8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

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