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验证数据展示

  • WB analysis of mouse liver using 28012-1-AP (same clone as 28012-1-PBS)

    WB analysis of mouse liver using 28012-1-AP (same clone as 28012-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

    IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZFPM1 antibody included
  • WB analysis using 28012-1-AP (same clone as 28012-1-PBS)

    WB analysis using 28012-1-AP (same clone as 28012-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

    IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZFPM1 antibody included

ZFPM1 Polyclonal antibody, PBS Only

ZFPM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28012-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZFPM1 Ready-To-Use IHC Kit. ZFPM1 primary antibody included.

产品信息

28012-1-PBS targets ZFPM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27829

Product name: Recombinant human ZFPM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-506 aa of NM_153813

Sequence: MTKANCERHLKVHTDTLSGVCHSCGFISTTRDILYSHLVTNHMVCQPGSKGEIYSPGAGHPATKLPPDSLGSFQQQHTALQGPLASADLGLAPTPSPGLDRKALAEATNGEARAEPLAQNGGSSEPPAAPRSIKVEAVEEPEAAPILGPGEPGPQAPSRTPSPRSPAPARVKAELSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein, multitype 1
别名
计算分子量 105 aa
观测分子量105 kDa
GenBank蛋白编号NM_153813
基因名称 ZFPM1
Gene ID (NCBI) 161882
RRIDAB_3086021
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IX07
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).

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