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验证数据展示

  • WB analysis of HepG2 using 27157-1-AP

    WB analysis of HepG2 using 27157-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27157-1-AP (ZFPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27157-1-AP

    IF Staining of HepG2 using 27157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFPL1 antibody (27157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ZFPL1 Polyclonal antibody

ZFPL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

zinc finger protein like 1, Zinc finger protein MCG4, Zinc finger protein-like 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27157-1-AP targets ZFPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24097

Product name: Recombinant human ZFPL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-264 aa of BC012814

Sequence: MGLCKCPKRKVTNLFCFEHRVNVCEHCLVANHAKCIVQSYLQWLQDSDYNPNCRLCNIPLASRETTRLVCYDLFHWACLNERAAQLPRNTAPAGYQCPSCNGPIFPPTNLAGPVASALREKLATVNWARAGLGLPLIDEVVSPEPEPLNTSDFSDWSSFNASSTPGPEEVDSASAAPAFYSQAPRPPASPGRPEQHTVIHMGNPEPLTHAPRKVYDTRDDDRTPGLHGDCDDDKYRRRPALGWLARLLRSRAGSRKRPLTLLQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein-like 1
别名 zinc finger protein like 1, Zinc finger protein MCG4, Zinc finger protein-like 1
观测分子量 35-40 kDa
GenBank蛋白编号BC012814
基因名称 ZFPL1
Gene ID (NCBI) 7542
RRIDAB_2880781
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95159
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ZFPL1 protein is a zinc finger protein localized to the Golgi apparatus, and its function is closely associated with maintaining Golgi structural integrity. Research indicates that ZFPL1 interacts with the Golgi matrix protein GM130 through its N-terminal domain, and this interaction is crucial for the proper anchoring of GM130 to the Golgi. When ZFPL1 expression is suppressed, GM130 dissociates from the membrane, leading to the fragmentation of the Golgi ribbon structure and significantly impairing protein secretion and transport processes. Therefore, ZFPL1 is considered a key scaffolding protein essential for maintaining the normal structure and function of the Golgi apparatus. Currently, its specific role in highly polarized cells with active secretory functions, such as neurons, is a major focus in the field.

实验方案

Product Specific Protocols
IF protocol for ZFPL1 antibody 27157-1-APDownload protocol
WB protocol for ZFPL1 antibody 27157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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