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验证数据展示

  • WB analysis of HepG2 using 27157-1-AP

    WB analysis of HepG2 using 27157-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27157-1-AP (ZFPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27157-1-AP

    IF Staining of HepG2 using 27157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFPL1 antibody (27157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ZFPL1 Polyclonal antibody

ZFPL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

D11S750, MCG4, ZFPL1, zinc finger protein like 1, Zinc finger protein MCG4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27157-1-AP targets ZFPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZFPL1 fusion protein Ag24097 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein-like 1
别名 D11S750, MCG4, ZFPL1, zinc finger protein like 1, Zinc finger protein MCG4
观测分子量 35-40 kDa
GenBank蛋白编号BC012814
基因名称 ZFPL1
Gene ID (NCBI) 7542
RRIDAB_2880781
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95159
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZFPL1 antibody 27157-1-APDownload protocol
IF protocol for ZFPL1 antibody 27157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols