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验证数据展示

  • IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

    IHC staining of human bladder using 12737-1-AP (same clone as 12737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZFP36 antibody included
  • IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

    IHC staining of human breast cancer using 12737-1-AP (same clone as 12737-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12737-1-AP (ZFP36 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZFP36 antibody included
  • WB analysis using 12737-1-AP (same clone as 12737-1-PBS)

    WB analysis using 12737-1-AP (same clone as 12737-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12737-1-AP (ZFP36 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12737-1-AP (same clone as 12737-1-PBS)

    IF Staining of HepG2 using 12737-1-AP (same clone as 12737-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36 antibody (12737-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12737-1-PBS in a different storage buffer formulation.

ZFP36 Polyclonal antibody, PBS Only

ZFP36 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12737-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZFP36 Ready-To-Use IHC Kit. ZFP36 primary antibody included.

产品信息

12737-1-PBS targets ZFP36 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3461

Product name: Recombinant human ZFP36 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-326 aa of BC009693

Sequence: MDLTAIYESLLSLSPDVPVPSDHGGTESSPGWGSSGPWSLSPSDSSPSGVTSRLPGRSTSLVEGRSCGWVPPPPGFAPLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRYGAKCQFAHGLGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHNPSEDLAAPGHPPVLRQSISFSGLPSGRRTSPPPPGLAGPSLSSSSFSPSSSPPPPGDLPLSPSAFSAAPGTPLARRDPTPVCCPSCRRATPISVWGPLGGLVRTPSVQSLGSDPDEYASSGSSLGGSDSPVFEAGVFAPPQPVAAPRRLPIFNRISVSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 36, C3H type, homolog (mouse)
别名 TTP, Zfp-36, Zfp 36, Tristetraprolin, mRNA decay activator protein ZFP36
计算分子量 326 aa, 34 kDa
观测分子量37-44 kDa
GenBank蛋白编号BC009693
基因名称 ZFP36
Gene ID (NCBI) 7538
RRIDAB_10598485
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26651
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The expression of many cytokines is regulated post-transcriptionally by factors that modulate mRNA transport, translation, and stability. Much of this regulation occurs by the binding and stabilizing, or destabilizing, of cytokine mRNAs by proteins that recognize adenosine and uridine-rich elements (AREs) in untranslated regions of target transcripts. Zfp36 is a mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. It was demonstrated to physically interact with the p65 subunit of nuclear factor-κB leading to decreased nuclear import and diminished transcriptional activation mediated by nuclear factor-κB. It acted by specifically binding ARE-containing mRNAs and promoting their degradation, and has a crucial role in the post-transcriptional regulation of tumor necrosis factor (TNF). The calcualted molecular weight of ZFP36 is 34 kDa, but modified ZFP36 is about 40-45 kDa.

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