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验证数据展示

  • WB analysis using 30834-1-AP

    WB analysis using 30834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30834-1-AP (ZFC3H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30834-1-AP

    IF Staining of U2OS using 30834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZFC3H1 antibody (30834-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of DU 145 using 30834-1-AP

    IP experiment of DU 145 using 30834-1-AP

    IP result of anti-ZFC3H1 (IP:30834-1-AP, 4ug; Detection:30834-1-AP 1:400) with DU 145 cells lysate 1360 ug.

ZFC3H1 Polyclonal antibody

ZFC3H1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 30834-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CCDC131, CSRC2, KIAA0546, PSRC2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells, HEK-293T cells
Positive IP detected inDU 145 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30834-1-AP targets ZFC3H1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34224

Product name: Recombinant human ZFC3H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1800-1989 aa of BC156732

Sequence: RNKVQEFKFFTDLVNRCLVTVPARYPIPFSSADYWSNYEFHNRVIFFYLSCVPKTQHSKTLERFCSVMPANSGLALRLLQHEWEESNVQILKLQAKMFTYNIPTCLATWKIAIAAEIVLKGQREVHRLYQRALQKLPLCASLWKDQLLFEASEGGKTDNLRKLVSKCQEIGVSLNELLNLNSNKTESKNH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, C3H1-type containing
别名 CCDC131, CSRC2, KIAA0546, PSRC2
计算分子量226 kDa
观测分子量250 kDa
GenBank蛋白编号BC156732
基因名称 ZFC3H1
Gene ID (NCBI) 196441
RRIDAB_3669762
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60293
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZFC3H1, also known as CCDC131 and PSRC2, is a large protein (1989 amino acids), with a C3H1- type zinc finger motif in its center, and contains five tetratricopeptide (TRP) repeats and six halftetratricopeptide (HAT) repeats at the C-terminal region. It is involved in the processing of a variety of RNAs and plays a critical role in the degradation of nuclear RNAs (PMID: 34514952).

实验方案

Product Specific Protocols
IF protocol for ZFC3H1 antibody 30834-1-APDownload protocol
IP protocol for ZFC3H1 antibody 30834-1-APDownload protocol
WB protocol for ZFC3H1 antibody 30834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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