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验证数据展示

  • WB analysis using 25048-1-AP (same clone as 25048-1-PBS)

    WB analysis using 25048-1-AP (same clone as 25048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25048-1-AP (ZFAND5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25048-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25048-1-AP (same clone as 25048-1-PBS)

    IP experiment of mouse brain using 25048-1-AP (same clone as 25048-1-PBS)

    IP result of anti-ZFAND5 (IP:25048-1-AP, 4ug; Detection:25048-1-AP 1:600) with mouse brain tissue lysate 1320 ug. This data was developed using the same antibody clone with 25048-1-PBS in a different storage buffer formulation.

ZFAND5 Polyclonal antibody, PBS Only

ZFAND5 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25048-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

AN1-type zinc finger protein 5, ZFAND5A, Zinc finger A20 domain-containing protein 2, ZNF216

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25048-1-PBS targets ZFAND5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19311

Product name: Recombinant human ZFAND5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 75-141 aa of BC027707

Sequence: NCEGAAGSTSEKSRNVPVAALPVTQQMTEMSISREDKITTPKTEVSEPVVTQPSPSVSQPSTSQSEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, AN1-type domain 5
别名 AN1-type zinc finger protein 5, ZFAND5A, Zinc finger A20 domain-containing protein 2, ZNF216
计算分子量 213 aa, 23 kDa
观测分子量 23-35 kDa
GenBank蛋白编号BC027707
基因名称 ZFAND5
Gene ID (NCBI) 7763
RRIDAB_3742134
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76080
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZFAND5 is Involved in protein degradation via the ubiquitin-proteasome system and may act by anchoring ubiquitinated proteins to the proteasome. It plays a role in ubiquitin-mediated protein degradation during muscle atrophy and in the regulation of NF-kappa-B activation and apoptosis. It can inhibit NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. It also inhibits tumor necrosis factor (TNF), IL-1, and TLR4-induced NF-kappa-B activation in a dose-dependent manner.

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