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验证数据展示

  • WB analysis using 16647-1-AP

    WB analysis using 16647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 16647-1-AP

    WB analysis of LNCaP using 16647-1-AP

    WB result of ZER1 antibody (16647-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZER1 transfected LNCaP cells.

  • IP experiment of mouse testis using 16647-1-AP

    IP experiment of mouse testis using 16647-1-AP

    IP result of anti-ZER1 (IP:16647-1-AP, 4ug; Detection:16647-1-AP 1:1000) with mouse testis tissue lysate 1680 ug.

  • Featured Product
  • KD/KO验证

ZER1 Polyclonal antibody

ZER1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16647-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

C9orf60, Hzyg, Protein zer-1 homolog, ZYG, Zyg-11 homolog B-like protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16647-1-AP targets ZER1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10031

Product name: Recombinant human ZER1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 324-766 aa of BC052563

Sequence: RPLQFLGLFENSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zer-1 homolog (C. elegans)
别名 C9orf60, Hzyg, Protein zer-1 homolog, ZYG, Zyg-11 homolog B-like protein
计算分子量 766 aa, 88 kDa
观测分子量 80-88 kDa
GenBank蛋白编号BC052563
基因名称 ZER1
Gene ID (NCBI) 10444
RRIDAB_2878294
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7L7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for ZER1 antibody 16647-1-APDownload protocol
WB protocol for ZER1 antibody 16647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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