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验证数据展示

  • WB analysis using 16647-1-AP

    WB analysis using 16647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 16647-1-AP

    IP experiment of mouse testis using 16647-1-AP

    IP result of anti-ZER1 (IP:16647-1-AP, 3ug; Detection:16647-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • IHC staining of human ovary using 16647-1-AP

    IHC staining of human ovary using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 16647-1-AP

    IHC staining of human testis using 16647-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

ZER1 Polyclonal antibody

ZER1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16647-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, rat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16647-1-AP targets ZER1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZER1 fusion protein Ag10031 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zer-1 homolog (C. elegans)
别名 C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL
计算分子量 766 aa, 88 kDa
观测分子量 80-88 kDa
GenBank蛋白编号BC052563
基因名称 ZER1
Gene ID (NCBI) 10444
RRIDAB_2878294
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7L7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZER1 antibody 16647-1-APDownload protocol
IHC protocol for ZER1 antibody 16647-1-APDownload protocol
IP protocol for ZER1 antibody 16647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols