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验证数据展示

  • WB analysis using 84803-4-RR (same clone as 84803-4-PBS)

    WB analysis using 84803-4-RR (same clone as 84803-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

    IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZDHHC5 antibody included
  • IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

    IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZDHHC5 antibody included
  • IP experiment of HEK-293 using 84803-4-RR (same clone as 84803-4-PBS)

    IP experiment of HEK-293 using 84803-4-RR (same clone as 84803-4-PBS)

    IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84803-4-RR

    Affinity and Kinetic Characterization of 84803-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

ZDHHC5 Recombinant monoclonal antibody, PBS Only

ZDHHC5 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 84803-4-PBS

产品说明书

CloneNo. 242192A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZDHHC5 Ready-To-Use IHC Kit. ZDHHC5 primary antibody included.

产品信息

84803-4-PBS targets ZDHHC5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15022

Product name: Recombinant human ZDHHC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 313-662 aa of BC026967

Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger, DHHC-type containing 5
别名 242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5
计算分子量 715 aa, 78 kDa
观测分子量 75-77 kDa
GenBank蛋白编号BC026967
基因名称 ZDHHC5
Gene ID (NCBI) 25921
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9C0B5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

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