验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32904-1-AP targets ZCWPW2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36666 Product name: Recombinant human ZCWPW2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-356 aa of BC094696 Sequence: VYSDDALSKENRVVCETEVLLKELEQMLQQALQPTATPDESEEGHGEEINMGEKLSKCSPEAPAGSLFENHYEEDYLVIDGIKLKAGECIEDITNKFKEIDALMSEF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, CW type with PWWP domain 2 |
| 别名 | Zinc Finger CW-Type And PWWP Domain 2, Zinc Finger CW-Type And PWWP Domain Containing 2 , Zinc finger CW-type PWWP domain protein 2, Zinc Finger, CW Type With PWWP Domain 2 |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC094696 |
| 基因名称 | ZCWPW2 |
| Gene ID (NCBI) | 152098 |
| RRID | AB_3742783 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q504Y3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZCWPW2 (Zinc Finger CW-type and PWWP Domain Containing 2) is a protein containing CW-type zinc finger and PWWP domains. It mainly serves as a histone methylation "reader" protein that binds lysine 4 (H3K4me0, H3K4me1, H3K4me3) in different methylation states on histone H3, and is involved in the regulation of chromatin structure and gene expression. In addition, ZCWPW2 protein plays an important role in meiosis and germ cell development, and its function is closely related to PRDM9 protein, which may be a key interacting protein in the mechanism of double-strand break repair or recombination.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCWPW2 antibody 32904-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
