验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, HEK-293T cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25196-1-AP targets ZCCHC6 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18240 Product name: Recombinant human ZCCHC6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC032456 Sequence: MGDTAKPYFVKRTKDRGTMDDDDFRRGHPQQDYLIIDDHAKGHGSKMEKGLQKKKITPGNYGNTPRKGPCAVSSNPYAFKNPIYSQPAWMNDSHKDQSKRWLSDEHTGNSDNWREFKPGPRIPVINRQRKDSFQENEDGYRWQDTRGCRTVRRLFHKDLTSLETTSEMEAGSPENKKQRSRPRKPRKTRNEENEQDGDLEGPVIDESVLSTKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCRLCDV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, CCHC domain containing 6 |
| 别名 | HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7, TUT7, TUTase 7, ZCCHC6 |
| 计算分子量 | 1495 aa, 171 kDa |
| 观测分子量 | 171 kDa |
| GenBank蛋白编号 | BC032456 |
| 基因名称 | ZCCHC6 |
| Gene ID (NCBI) | 79670 |
| RRID | AB_2879953 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VYS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ZCCHC6 antibody 25196-1-AP | Download protocol |
| WB protocol for ZCCHC6 antibody 25196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Mol Cell 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
| ||
Nat Commun AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2. | ||
Elife The molecular basis of coupling between poly(A)-tail length and translational efficiency. |
