验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18980-1-AP targets ZCCHC11 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, CCHC domain containing 11 |
| 别名 | EC:2.7.7.52, KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4 |
| 计算分子量 | 185 kDa |
| 观测分子量 | 185 kDa |
| GenBank蛋白编号 | NM_015269 |
| 基因名称 | ZCCHC11 |
| Gene ID (NCBI) | 23318 |
| RRID | AB_10598327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5TAX3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| IP protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| WB protocol for ZCCHC11 antibody 18980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.
| ||
Nature A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.
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Cell Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.
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Mol Cell 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.
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