验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20526-1-AP targets ZC4H2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14388 Product name: Recombinant human ZC4H2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC004411 Sequence: MADEQEIMCKLESIKEIRNKTLQMEKIKARLKAEFEALESEERHLKEYKQEMDLLLQEKMAHVEELRLIHADINVMENTIKQSENDLNKLLESTRRLHDEYKPLKEHVDALRMTLGLQRLPDLCEEEEKLSLDYFEKQKAEWQTEPQEPPIPESLAAAAAAAQQLQVARKQDTRQTATFRQQPPPMKACLSCHQQIHRNAPICPLCKAKSRSRNPKKPKRKQDE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, C4H2 domain containing |
| 别名 | HCA127, Hepatocellular carcinoma-associated antigen 127, KIAA1166, Zinc finger C4H2 domain-containing protein |
| 计算分子量 | 224 aa, 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC004411 |
| 基因名称 | ZC4H2 |
| Gene ID (NCBI) | 55906 |
| RRID | AB_3742000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQZ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZC4H2 is located on the X chromosome, which encodes a C4H2 type zinc-finger nuclear factor. Mutations in ZC4H2 have been reported to be associated with various disorders, including arthrogryposis multiplex congenita (AMC), X-linked intellectual disability, Wieacker-Wolff syndrome, and Miles-Carpenter syndrome . Since ZC4H2 is located on the X-chromosome, the disorder is usually severe or even lethal in males. Mechanistically, ZC4H2 has been shown mostly to work as a stabilizing co-factor of the ubiquitin E3 ligase RNF220. RNF220 and ZC4H2 cooperate to pattern the embryonic ventral neural tube through regulation of Shh/Gli signaling as well as the stability of key transcription factors (Dbx1/2 and Nkx2.2) . (PMID: 31336385, PMID: 39336725)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC4H2 antibody 20526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
