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验证数据展示

  • WB analysis of K-562 using 26241-1-AP

    WB analysis of K-562 using 26241-1-AP

    WB result of ZC3H15 antibody (26241-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H15 transfected K-562 cells.

  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • WB analysis of HeLa using 26241-1-AP

    WB analysis of HeLa using 26241-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 26241-1-AP

    IF Staining of U2OS using 26241-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ZC3H15 antibody (26241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 26241-1-AP

    IF Staining of HeLa using 26241-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26241-1-AP (ZC3H15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

ZC3H15 Polyclonal antibody

ZC3H15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26241-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H15 Ready-To-Use IHC Kit. ZC3H15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26241-1-AP targets ZC3H15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag24091

Product name: Recombinant human ZC3H15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-426 aa of BC021102

Sequence: ELQELNELFKPVVAAQKISKGADPKSVVCAFFKQGQCTKGDKCKFSHDLTLERKCEKRSVYIDARDEELEKDTMDNWDEKKLEEVVNKKHGEAEKKKPKTQIVCKHFLEAIENNKYGWFWVCPGGGDICMYRHALPPGFVLKKDKKKEEKEDEISLEDLIERERSALGPNVTKITLESFLAWKKRKRQEKIDKLEQDMERRKADFKAGKALVISGREVFEFRPELVNDDDEEADDTRYTQGTGGDEVDDSVSVNDIDLSLYIPRDVDETGITVASLERFSTYTSDKDENKLSEASGGRAENGERSDLEEDNEREGTENGAIDAVPVDENLFTGEDLDELEEELNTLDLEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger CCCH-type containing 15
别名 DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15
观测分子量 50-55 kDa
GenBank蛋白编号BC021102
基因名称 ZC3H15
Gene ID (NCBI) 55854
RRIDAB_2880441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ZC3H15 antibody 26241-1-APDownload protocol
IHC protocol for ZC3H15 antibody 26241-1-APDownload protocol
WB protocol for ZC3H15 antibody 26241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Dis

ZC3H15 regulates the ubiquitination of PTEN via recruitment of TRIM56 and promotes malignant progression of non-small cell lung cancer.

Authors - Peihong Wu
View Article
humanWB

Cell Biosci

Disruption of ZC3H15 compromises telomere length maintenance by entrapping telomerase within cajal bodies

Authors - Chuanle Wang
  • KD Validated
View Article
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