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验证数据展示

  • WB analysis of K-562 using 26241-1-AP

    WB analysis of K-562 using 26241-1-AP

    WB result of ZC3H15 antibody (26241-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H15 transfected K-562 cells.

  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • WB analysis of HeLa using 26241-1-AP

    WB analysis of HeLa using 26241-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 26241-1-AP

    IF Staining of U2OS using 26241-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ZC3H15 antibody (26241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 26241-1-AP

    IF Staining of HeLa using 26241-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26241-1-AP (ZC3H15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ZC3H15 Polyclonal antibody

ZC3H15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26241-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H15 Ready-To-Use IHC Kit. ZC3H15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26241-1-AP targets ZC3H15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24091

Product name: Recombinant human ZC3H15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 77-426 aa of BC021102

Sequence: ELQELNELFKPVVAAQKISKGADPKSVVCAFFKQGQCTKGDKCKFSHDLTLERKCEKRSVYIDARDEELEKDTMDNWDEKKLEEVVNKKHGEAEKKKPKTQIVCKHFLEAIENNKYGWFWVCPGGGDICMYRHALPPGFVLKKDKKKEEKEDEISLEDLIERERSALGPNVTKITLESFLAWKKRKRQEKIDKLEQDMERRKADFKAGKALVISGREVFEFRPELVNDDDEEADDTRYTQGTGGDEVDDSVSVNDIDLSLYIPRDVDETGITVASLERFSTYTSDKDENKLSEASGGRAENGERSDLEEDNEREGTENGAIDAVPVDENLFTGEDLDELEEELNTLDLEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger CCCH-type containing 15
别名 DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15
观测分子量 50-55 kDa
GenBank蛋白编号BC021102
基因名称 ZC3H15
Gene ID (NCBI) 55854
RRIDAB_2880441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZC3H15 antibody 26241-1-APDownload protocol
IHC protocol for ZC3H15 antibody 26241-1-APDownload protocol
IF protocol for ZC3H15 antibody 26241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols