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验证数据展示

  • WB analysis of HEK-293 using 68526-1-Ig

    WB analysis of HEK-293 using 68526-1-Ig

    WB result of ZC3H13 antibody (68526-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H13 transfected HEK-293 cells.

  • WB analysis using 68526-1-Ig

    WB analysis using 68526-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68526-1-Ig (ZC3H13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 68526-1-Ig

    IHC staining of human stomach cancer using 68526-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68526-1-Ig (ZC3H13 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H13 antibody included
  • Featured Product
  • KD/KO验证

ZC3H13 Monoclonal antibody

ZC3H13 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68526-1-Ig

产品说明书

CloneNo. 3E8C5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0853, 3E8C5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, HEK-293 cells, A549 cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZC3H13 Ready-To-Use IHC Kit. ZC3H13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68526-1-Ig targets ZC3H13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29132

Product name: Recombinant human ZC3H13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of NM_001076788

Sequence: MSKIRRKVTVENTKTISDSTSRRPSVFERLGPSTGSTAETQCRNWLKTGNCLYGNTCRFVHGPSPRGKGYSSNYRRSPERPTGDLRERMKNKRQDVDTEPQKRNTEESSSPVRKESSRGRHREKEDIKITKERTPESEEENVEWETNRDDSDNGDINYDYVHELSLEMKRQKIQRELMKLEQENMEKREEIIIKKEVSPEVVRSKLSPSPSLRKSSKSPKRKSSPKSSSASKKDRKTSAV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger CCCH-type containing 13
别名 KIAA0853, 3E8C5
计算分子量 197 kDa
观测分子量196-240 kDa
GenBank蛋白编号NM_001076788
基因名称 ZC3H13
Gene ID (NCBI) 23091
RRIDAB_3085234
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5T200
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ZC3H13 antibody 68526-1-IgDownload protocol
WB protocol for ZC3H13 antibody 68526-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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