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ZBTB8A Polyclonal antibody

ZBTB8A Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24544-1-AP

Print datasheet

Synonyms

BOZ F1, BOZF1, ZBTB8, ZBTB8A

验证数据展示

  • WB analysis of HEK-293 using 24544-1-AP

    WB analysis of HEK-293 using 24544-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24544-1-AP (ZBTB8A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24544-1-AP

    IP experiment of HEK-293 using 24544-1-AP

    IP result of anti-ZBTB8A (IP:24544-1-AP, 3ug; Detection:24544-1-AP 1:300) with HEK-293 cells lysate 920ug.

  • IF Staining of HEK-293 using 24544-1-AP

    IF Staining of HEK-293 using 24544-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24544-1-AP (ZBTB8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24544-1-AP is ZBTB8A Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human
Immunogen ZBTB8A fusion protein Ag21588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 8A
Synonyms BOZ F1, BOZF1, ZBTB8, ZBTB8A
Calculated Molecular Weight 441 aa, 50 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC015239
Gene Symbol ZBTB8A
Gene ID (NCBI) 653121
RRIDAB_2879599
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BR9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB8A, also named as Zinc finger and BTB domain-containing protein 8A, is a 441 amino acid protein, which may be involved in transcriptional regulation. ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis. The calcualted molecular weight of ZBTB8A is 50 kDa, but modified ZBTB8A is about 55-60 kDa.

实验方案

Product Specific Protocols
WB protocol for ZBTB8A antibody 24544-1-APDownload protocol
IF protocol for ZBTB8A antibody 24544-1-APDownload protocol
IP protocol for ZBTB8A antibody 24544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols