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验证数据展示

  • WB analysis using 30658-1-AP

    WB analysis using 30658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30658-1-AP (ZBTB7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 30658-1-AP

    IHC staining of human lung using 30658-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30658-1-AP (ZBTB7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 30658-1-AP

    IHC staining of human lung using 30658-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30658-1-AP (ZBTB7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 30658-1-AP

    IF Staining of MCF-7 using 30658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB7A antibody (30658-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of HeLa using 30658-1-AP

    IF Staining of HeLa using 30658-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7A antibody (30658-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

ZBTB7A Polyclonal antibody

ZBTB7A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30658-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Factor binding IST protein 1, FBI 1, FBI1, FBI-1, HIV 1 1st binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, PC-3 cells, SGC-7901 cells, U2OS cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30658-1-AP targets ZBTB7A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZBTB7A fusion protein Ag31077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 7A
别名 Factor binding IST protein 1, FBI 1, FBI1, FBI-1, HIV 1 1st binding protein 1
计算分子量 61 kDa
观测分子量61-70 kDa
GenBank蛋白编号NM_015898
基因名称 ZBTB7A
Gene ID (NCBI) 51341
RRIDAB_3669747
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa.

实验方案

Product Specific Protocols
WB protocol for ZBTB7A antibody 30658-1-APDownload protocol
IHC protocol for ZBTB7A antibody 30658-1-APDownload protocol
IF protocol for ZBTB7A antibody 30658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols