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验证数据展示

  • IHC staining of mouse skin using 25455-1-AP

    IHC staining of mouse skin using 25455-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25455-1-AP (ZBTB46 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 25455-1-AP

    IHC staining of mouse skin using 25455-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25455-1-AP (ZBTB46 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of RAW 264.7 using 25455-1-AP

    WB analysis of RAW 264.7 using 25455-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25455-1-AP (ZBTB46 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ZBTB46 Polyclonal antibody

ZBTB46 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25455-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Zinc finger and BTB domain-containing protein 46, RINZF, BZEL, BTB-ZF protein expressed in effector lymphocytes, BTBD4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25455-1-AP targets ZBTB46 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22273

Product name: Recombinant human ZBTB46 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-416 aa of BC073800

Sequence: ELAEFEIGASSSSSTEALISAVMAGRSISPWLARRTSPANSSGDSAIASCHDGGSSYGKEDQEPKADGPDDVSSQPLWPGDVGYGPLRIKEEQVSPSQYGGSELPSAKDGAVQNSFSEQSAGDAWQPTGRRKNRKNKETVRHITQQVEDDSRASSPVPSFLPTSGWPFSSRDSNADLSVTEASSSDSRGERAELYAQVEEGLLGGEASYLGPPLTPEKDDALHQATAVANLRAALMSKNSLLSLKADVLGDDGSLLFEYLPRGAHSLSLNEFTVIRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 46
别名 Zinc finger and BTB domain-containing protein 46, RINZF, BZEL, BTB-ZF protein expressed in effector lymphocytes, BTBD4
计算分子量 589 aa, 64 kDa
观测分子量 70 kDa
GenBank蛋白编号BC073800
基因名称 ZBTB46
Gene ID (NCBI) 140685
RRIDAB_2880088
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UZ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB46, also named as BTBD4, ZNF340, is a 589 amino acid protein, which contains one BTB (POZ) domain and two C2H2-type zinc fingers. ZBTB46 functions as a transcriptional repressor for PRDM1.

实验方案

Product Specific Protocols
IHC protocol for ZBTB46 antibody 25455-1-APDownload protocol
WB protocol for ZBTB46 antibody 25455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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