验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, DU 145 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human testis tissue, human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
21906-1-AP targets ZBTB38 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14963 Product name: Recombinant human ZBTB38 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 846-1195 aa of BC008901 Sequence: EAIVKRHILGSKLFYKRGRRPKYQMQEEPLPQGNDPEPSGDSPLGLCQSECMEMSEVFDDASDQDSTDKPWRPYYNYKPKKKSRQLKKMRKVNWRKEHGNRSPSHKCKYPAELDCAVGKAPQDKPFEEEETKEMPKLQCELCDGDKAVGAGNQGRPHRHLTSRPYACELCAKQFQSPSTLKMHMRCHTGEKPYQCKTCGRCFSVQGNLQKHERIHLGLKEFVCQYCNKAFTLNETLKIHERIHTGEKRYHCQFCFQRFLYLSTKRNHEQRHIREHNGKGYACFQCPKICKTAAALGMHQKKHLFKSPSQQEKIGDVCHENSNPLENQHFIGSEDNDQKDNIQTGVENVVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger and BTB domain containing 38 |
| 别名 | CIBZ, ZBTB38 |
| 计算分子量 | 1195 aa, 134 kDa |
| 观测分子量 | 134 kDa |
| GenBank蛋白编号 | BC008901 |
| 基因名称 | ZBTB38 |
| Gene ID (NCBI) | 253461 |
| RRID | AB_2878943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NAP3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZBTB38 antibody 21906-1-AP | Download protocol |
| WB protocol for ZBTB38 antibody 21906-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med Cisplatin resistance-related multi-omics differences and the establishment of machine learning models. | ||
Cell Death Dis ZBTB38 suppresses prostate cancer cell proliferation and migration via directly promoting DKK1 expression. | ||
J Gynecol Oncol Prognostication of early-onset endometrioid endometrial cancer based on genome-wide DNA methylation profiles |
