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验证数据展示

  • IHC staining of mouse testis using 12516-1-AP

    IHC staining of mouse testis using 12516-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 12516-1-AP

    WB analysis using 12516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12516-1-AP (ZBTB32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 12516-1-AP

    IF Staining of MCF-7 using 12516-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 21738-1-AP 00069062 (red).

ZBTB32 Polyclonal antibody

ZBTB32 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12516-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

ZNF538, Zinc finger protein 538, Zinc finger and BTB domain-containing protein 32, FAZF, FAXF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue, pig lymph node tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12516-1-AP targets ZBTB32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag3199

Product name: Recombinant human ZBTB32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-302 aa of BC017700

Sequence: MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQKPEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQRRTCNLCGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 32
别名 ZNF538, Zinc finger protein 538, Zinc finger and BTB domain-containing protein 32, FAZF, FAXF
计算分子量 487 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017700
基因名称 ZBTB32
Gene ID (NCBI) 27033
RRIDAB_3669143
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Y4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The zinc finger and BTB domain containing 32 (ZBTB32) protein, also known as testis zinc finger protein (TZFP) or repressor of GATA (ROG), is a transcription factor belonging to the BTB/POZ-ZF protein family. It is encoded by the Zbtb32 gene, located on proximal chromosome 7 in the mouse genome. ZBTB32 was shown to ensure proper regulation of meiosis during spermatogenesis by acting as a repressor of the androgen receptor (PMID: 22851713, 34337361).

实验方案

Product Specific Protocols
WB protocol for ZBTB32 antibody 12516-1-APDownload protocol
IHC protocol for ZBTB32 antibody 12516-1-APDownload protocol
IF protocol for ZBTB32 antibody 12516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols