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ZBTB25 Polyclonal antibody

ZBTB25 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25631-1-AP

Print datasheet

Synonyms

C14orf51, KUP, ZBTB25, Zinc finger protein 46, Zinc finger protein KUP, ZNF46

验证数据展示

  • WB analysis of mouse testis using 25631-1-AP

    WB analysis of mouse testis using 25631-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25631-1-AP (ZBTB25 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of HepG2 using 25631-1-AP

    IF Staining of HepG2 using 25631-1-AP

    Immunofluorescent analysis of HepG2 cells using 25631-1-AP (ZBTB25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue, mouse spleen tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25631-1-AP is ZBTB25 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ZBTB25 fusion protein Ag22507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 25
Synonyms C14orf51, KUP, ZBTB25, Zinc finger protein 46, Zinc finger protein KUP, ZNF46
Calculated Molecular Weight 435 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC035804
Gene Symbol ZBTB25
Gene ID (NCBI) 7597
RRIDAB_2880171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZBTB25 antibody 25631-1-APDownload protocol
IF protocol for ZBTB25 antibody 25631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article