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验证数据展示

  • WB analysis of HeLa using 26287-1-AP

    WB analysis of HeLa using 26287-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26287-1-AP (ZBTB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26287-1-AP

    IP experiment of HeLa using 26287-1-AP

    IP result of anti-ZBTB1 (IP:26287-1-AP, 4ug; Detection:26287-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IF Staining of HeLa using 26287-1-AP

    IF Staining of HeLa using 26287-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26287-1-AP (ZBTB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ZBTB1 Polyclonal antibody

ZBTB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 26287-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA

别名

KIAA0997, ZBTB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26287-1-AP targets ZBTB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag23492

Product name: Recombinant human ZBTB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-357 aa of BC050719

Sequence: MAKPSHSSYVLQQLNNQREWGFLCDCCIAIDDIYFQAHKAVLAACSSYFRMFFMNHQHSTAQLNLSNMKISAECFDLILQFMYLGKIMTAPSSFEQFKVAMNYLQLYNVPDCLEDIQDADCSSSKCSSSASSKQNSKMIFGVRMYEDTVARNGNEANRWCAEPSSTVNTPHNREADEESLQLGNFPEPLFDVCKKSSVSKLSTPKERVSRRFGRSFTCDSCGFGFSCEKLLDEHVLTCTNRHLYQNTRSYHRIVDIRDGKDSNIKAEFGEKDSSKTFSAQTDKYRGDTSQAADDSASTTGSRKSSTVESEIASEEKSRAAERKRIIIKMEPEDTPTDELKDFNIIKVTDKDCNESTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 1
别名 KIAA0997, ZBTB1
观测分子量 82 kDa
GenBank蛋白编号BC050719
基因名称 ZBTB1
Gene ID (NCBI) 22890
RRIDAB_2880463
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZBTB1 antibody 26287-1-APDownload protocol
IF protocol for ZBTB1 antibody 26287-1-APDownload protocol
IP protocol for ZBTB1 antibody 26287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Authors - Robert T Williams
  • KO Validated
View Article
mouseWB

iScience

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner
View Article