验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat brain tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23958-1-AP targets ZBBX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag20988 Product name: Recombinant human ZBBX protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-326 aa of BC034229 Sequence: MEKVQLEFENQEMEKKLQEFRSTRNKEKEDRESSEYYWKSGKVGKLVNQSYMMSQNKGNVVKFSAGKVKLKLLKEQIQEPVKPTVNYKMANSSECEKPKINGKVCGQCENKAALLVCLECGEDYCSGCFANVHQKGALKLHRTTLLQARSQILFNVLDVAHQFIKDVNPDEPKEENNSTKETSKIQHKPKSVLLQRSSSEVEITTMKRAQRTKPRKSLLCEGSFDEEASAQSFQEVLSQWRTGNHDDNKKQNLHAAVKDSLEECEVQTNLKIWREPLNIELKEDILSYMEKLWLKKHRRTPQEQLFKCYQIRSHIHMKPLVMHSVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, B-box domain containing |
| 别名 | Zinc finger B box domain containing protein 1 |
| 计算分子量 | 800 aa, 91 kDa |
| 观测分子量 | 88-96 kDa |
| GenBank蛋白编号 | BC034229 |
| 基因名称 | ZBBX |
| Gene ID (NCBI) | 79740 |
| RRID | AB_3742099 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | A8MT70 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZBBX (Zinc finger B-box domain-containing protein 1) contains a classic B-box-type zinc-finger (B-box1) motif that adopts a C6H2 zinc-binding fold and is therefore predicted to bind zinc ions and mediate protein-protein or protein-nucleic acid interactions. ZBBX was implicated previously by a de novo deletion in a male ADHD proband, and separately by a CNV gain disrupting ZBBX (PMID: 21832240).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZBBX antibody 23958-1-AP | Download protocol |
| WB protocol for ZBBX antibody 23958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
