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YTHDF1 Polyclonal antibody
YTHDF1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF-P, IP, ELISA and More (3)
Conjugate
Unconjugated
222
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, A549 cells, mouse brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human kidney tissue, human hypothalamus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17479-1-AP targets YTHDF1 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, zebrafish |
Immunogen | YTHDF1 fusion protein Ag11509 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | YTH domain family, member 1 |
Synonyms | YTH domain-containing family protein 1, DF1, DACA-1, C20orf21 |
Calculated Molecular Weight | 559 aa, 61 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC050284 |
Gene Symbol | YTHDF1 |
Gene ID (NCBI) | 54915 |
RRID | AB_2217473 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BYJ9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
IHC protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
IF protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
IP protocol for YTHDF1 antibody 17479-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature m6A facilitates hippocampus-dependent learning and memory through YTHDF1.
| ||
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Cell A Unified Model for the Function of YTHDF Proteins in Regulating m6A-Modified mRNA.
| ||
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. |