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验证数据展示

  • WB analysis of SH-SY5Y using 21332-1-AP

    WB analysis of SH-SY5Y using 21332-1-AP

    WB result of YLPM1 antibody (21332-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YLPM1 transfected SH-SY5Y cells.

  • WB analysis using 21332-1-AP

    WB analysis using 21332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21332-1-AP (YLPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Neuro-2a using 21332-1-AP

    IP experiment of Neuro-2a using 21332-1-AP

    IP result of anti-YLPM1 (IP:21332-1-AP, 4ug; Detection:21332-1-AP 1:1000) with Neuro-2a cells lysate 1440 ug.

  • IF Staining of SH-SY5Y using 21332-1-AP

    IF Staining of SH-SY5Y using 21332-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using YLPM1 antibody (21332-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • KD/KO Validated

YLPM1 Polyclonal antibody

YLPM1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 21332-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

C14orf170, YLP motif-containing protein 1, YLPM 1, ZAP, ZAP113

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-251 cells, Neuro-2a cells, C6 cells
Positive IP detected inNeuro-2a cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21332-1-AP targets YLPM1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15408

Product name: Recombinant human YLPM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1960-2146 aa of BC023570

Sequence: MSADNQTCGKRNIHGRKLKEINKMADHWETAPRHMMRLDIRSLLQDAAIEEVEMEDFDANIEEQKEEKKDAEEEESELGYIPKSKWEMDTSEAKLDKLDGLRTGTKRKRDWEAIASRMEDYLQLPDDYDTRASEPGKKRVRWADLEEKKDADRKRAIGFVVGQTDWEKITDESGHLAEKALNRTKYI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YLP motif containing 1
别名 C14orf170, YLP motif-containing protein 1, YLPM 1, ZAP, ZAP113
计算分子量 1951 aa, 220 kDa
观测分子量200 kDa, 250-290 kDa
GenBank蛋白编号BC023570
基因名称 YLPM1
Gene ID (NCBI) 56252
RRIDAB_3742028
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YLPM1 also known as ZAP3, ZAP113, or C14orf170. YLPM1 plays a critical role in the regulation of telomerase activity and transcriptional repression. The molecular weight of the human YLPM1 protein is approximately 250-300 kDa, with a shorter isoform about 200 kDa.

实验方案

Product Specific Protocols
IF protocol for YLPM1 antibody 21332-1-APDownload protocol
IP protocol for YLPM1 antibody 21332-1-APDownload protocol
WB protocol for YLPM1 antibody 21332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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