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验证数据展示

  • WB analysis using 12931-1-AP

    WB analysis using 12931-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12931-1-AP (YIPF5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 12931-1-AP

    IHC staining of human ovary cancer using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

YIPF5 Polyclonal antibody

YIPF5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12931-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

FINGER5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, MEDS2, PP12723, Protein YIPF5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12931-1-AP targets YIPF5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3802

Product name: Recombinant human YIPF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-131 aa of BC024737

Sequence: MSGFENLNTDFYQTSYSIDDQSQQSYDYGGSGGPYSKQYAGYDYSQQGRFVPPDMMQPQQPYTGQIYQPTQAYTPASPQPFYGNNFEDEPPLLEELGINFDHIWQKTLTVLHPLKVADGSIMNETDLAGPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Yip1 domain family, member 5
别名 FINGER5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, MEDS2, PP12723, Protein YIPF5
计算分子量 257 aa, 28 kDa
观测分子量 28-33 kDa
GenBank蛋白编号BC024737
基因名称 YIPF5
Gene ID (NCBI) 81555
RRIDAB_2217209
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for YIPF5 antibody 12931-1-APDownload protocol
IHC protocol for YIPF5 antibody 12931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Front Pharmacol

Salidroside enhances 5-fluorouracil sensitivity against hepatocellular carcinoma via YIPF5-induced mitophagy

Authors - Sumin Sun
View Article