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验证数据展示

  • WB analysis using 12931-1-AP

    WB analysis using 12931-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12931-1-AP (YIPF5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 12931-1-AP

    IHC staining of human ovary cancer using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

YIPF5 Polyclonal antibody

YIPF5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

FINGER5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, MEDS2, PP12723, Protein YIPF5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12931-1-AP targets YIPF5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YIPF5 fusion protein Ag3802 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Yip1 domain family, member 5
别名 FINGER5, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5, MEDS2, PP12723, Protein YIPF5
计算分子量 257 aa, 28 kDa
观测分子量 28-33 kDa
GenBank蛋白编号BC024737
基因名称 YIPF5
Gene ID (NCBI) 81555
RRIDAB_2217209
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for YIPF5 antibody 12931-1-APDownload protocol
IHC protocol for YIPF5 antibody 12931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols