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验证数据展示

  • WB analysis of HepG2 using 20441-1-AP

    WB analysis of HepG2 using 20441-1-AP

    WB result of YIPF2 antibody (20441-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YIPF2 transfected HepG2 cells.

  • IP experiment of HepG2 using 20441-1-AP

    IP experiment of HepG2 using 20441-1-AP

    IP result of anti-YIPF2 (IP:20441-1-AP, 4ug; Detection:20441-1-AP 1:500) with HepG2 cells lysate 1470 ug.

  • WB analysis using 20441-1-AP

    WB analysis using 20441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20441-1-AP (YIPF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

YIPF2 Polyclonal antibody

YIPF2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

FinGER2, Protein YIPF2, Yip1 domain family, member 2, YIP1 family member 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20441-1-AP targets YIPF2 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14238

Product name: Recombinant human YIPF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-86 aa of BC000056

Sequence: MASADELTFHEFEEATNLLADTPDAATTSRSDQLTPQGHVAVAVGSGGSYGAEDEVEEESDKAALLQEQQQQQQPGFWTFSYYQSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Yip1 domain family, member 2
别名 FinGER2, Protein YIPF2, Yip1 domain family, member 2, YIP1 family member 2
计算分子量 316 aa, 35 kDa
观测分子量35~40 kDa
GenBank蛋白编号BC000056
基因名称 YIPF2
Gene ID (NCBI) 78992
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YIPF2 is a member of YIP family whose name refers to the Ypt (yeast RAB GTPase)-interacting protein predicted to have five transmembrane segments with an N-terminal exposed to the cytoplasm and a short C-terminal exposed to the lumen of the secretory pathway. YIPF2 has been reported to mainly locate in the trans-Golgi network (TGN) co-localized with virous RAB proteins, suggesting that it is potentially involved in vesicle transport (PMID: 31189879). YIPF2 can serve as the GDF (GDI-displacement factor) of RAB5/RAB22A and intracellular binder of CD147 in HCC cells (PMID: 311898799).

实验方案

Product Specific Protocols
IP protocol for YIPF2 antibody 20441-1-APDownload protocol
WB protocol for YIPF2 antibody 20441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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