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验证数据展示

  • WB analysis using 67196-1-Ig

    WB analysis using 67196-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67196-1-Ig

    WB analysis of HepG2 using 67196-1-Ig

    WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells.

  • IHC staining of human kidney using 67196-1-Ig

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included
  • IHC staining of human kidney using 67196-1-Ig

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included
  • Featured Product
  • KD/KO验证

YES1 Monoclonal antibody

YES1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67196-1-Ig

产品说明书

CloneNo. 1A12E7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat

应用

WB, IHC, ELISA

别名

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, LNCaP cells, A431 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67196-1-Ig targets YES1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag5016

Product name: Recombinant human YES1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 275-526 aa of BC048960

Sequence: ESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
别名 c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1
计算分子量 61 kDa
观测分子量 62 kDa
GenBank蛋白编号BC048960
基因名称 YES1
Gene ID (NCBI) 7525
RRIDAB_2918483
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07947
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

实验方案

Product Specific Protocols
WB protocol for YES1 antibody 67196-1-IgDownload protocol
IHC protocol for YES1 antibody 67196-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols