验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24717-1-AP targets YEATS2 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20134 Product name: Recombinant human YEATS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1066-1422 aa of BC150273 Sequence: VQTILMPVNKVVQSFSTSKPPAILPVAAPTPVVPSSAPAAVAKVKTEPETPGPSCLSQEGQTAVKTEESSELGNYVIKIDHLETIQQLLTAVVKKIPLITAKSEDASCFSAKSVEQYYGWNIGKRRAAEWQRAMTMRKVLQEILEKNPRFHHLTPLKTKHIAHWCRCHGYTPPDPESLRNDGDSIEDVLTQIDSEPECPSSFSSADNLCRKLEDLQQFQKREPENEEEVDILSLSEPVKINIKKEQEEKQEEVKFYLPPTPGSEFIGDVTQKIGITLQPVALHRNVYASVVEDMILKATEQLVNDILRQALAVGYQTASHNRIPKEITVSNIHQAICNIPFLDFLTNKHMGILNEDQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | YEATS domain containing 2 |
| 别名 | KIAA1197, YEATS domain containing 2, YEATS domain-containing protein 2 |
| 计算分子量 | 1422 aa, 151 kDa |
| 观测分子量 | 151 kDa |
| GenBank蛋白编号 | BC150273 |
| 基因名称 | YEATS2 |
| Gene ID (NCBI) | 55689 |
| RRID | AB_2879686 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULM3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YEATS domain containing 2 (YEATS2) protein is a scaffolding subunit of the ATAC complex. Studies have shown that YEATS2 is a selective histone crotonylation reader and is related to histone acetylation. In addition, YEATS2 is associated with the progression of various tumors. Overexpression of YEATS2 promotes the proliferation and migration of pancreatic cancer cells. YEATS2 has been reported to be associated with the progression of non-small-cell lung cancer through histone acetylation.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for YEATS2 antibody 24717-1-AP | Download protocol |
| WB protocol for YEATS2 antibody 24717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma. | ||
Nat Commun YEATS2 links histone acetylation to tumorigenesis of non-small cell lung cancer.
| ||
Nat Chem Biol Complex-specific inhibitors for interrogating ATAC histone acetyltransferase complex. | ||
Cell Death Differ Matrix stiffness-induced YEATS2 drives HCC progression via epigenetic activation of the TGFBR2-TAZ-AKT pathway.
| ||
Phytomedicine Cinobufacini retards progression of pancreatic ductal adenocarcinoma through targeting YEATS2/TAK1/NF-κB axis | ||
Oncogene YEATS2 promotes DNA repair and induces anoikis resistance by enhancing chromatin accessibility to drive prostate cancer metastasis. |
