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验证数据展示

  • IF Staining of HeLa using CL488-84993-4

    IF Staining of HeLa using CL488-84993-4

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 YBX1 antibody (CL488-84993-4, Clone: 242462F12 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated YBX1 Recombinant antibody

YBX1 Recombinant Antibody for IF/ICC
Cat No. CL488-84993-4

产品说明书

CloneNo. 242462F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

242462F12, CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy YBX1 Ready-To-Use IHC Kit. YBX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84993-4-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84993-4 targets YBX1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 YBX1 fusion protein Ag14129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Y box binding protein 1
别名 242462F12, CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB
计算分子量 324 aa, 36 kDa
观测分子量 36-56 kDa
GenBank蛋白编号BC010430
基因名称 YBX1
Gene ID (NCBI) 4904
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP67809
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also, it involves the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. The calculated molecular weight of YBX1 is 36 kDa, but modified YBX1 is about 36-56 kDa. (PMID: 27384875 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YBX1 antibody CL488-84993-4Download protocol
Standard Protocols
Click here to view our Standard Protocols