验证数据展示
经过测试的应用
| Positive IP detected in | MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55064-1-AP targets XYLT2 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | xylosyltransferase II |
| 别名 | Peptide O xylosyltransferase 1, Xylosyltransferase 2, xylT II |
| 计算分子量 | 97 kDa |
| 观测分子量 | 97-110 kDa |
| GenBank蛋白编号 | NM_022167 |
| 基因名称 | XYLT2 |
| Gene ID (NCBI) | 64132 |
| RRID | AB_3743198 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1B5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Xylosyltransferases catalyze the attachment of xylose, first linker sugar residue, to a major group of extracellular matrix proteins, namely proteoglycans, and are therefore of key importance for proper PG biosynthesis in many tissues (PMID: 26027496). XYLT2 (xylosyltransferase II, XylT-II) is a protein homologous to XYLT1 but with hitherto unknown activity or physiological function. In most tissues, both xylosyltransferase genes are expressed and in numerous cell lines XYLT2 is the major xylosyltransferase (PMID: 17189265, 25748573).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for XYLT2 antibody 55064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
