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XYLT1 Polyclonal antibody

XYLT1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 55061-1-AP

Print datasheet

Synonyms

Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1

验证数据展示

  • WB analysis of HEK-293 using 55061-1-AP

    WB analysis of HEK-293 using 55061-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 55061-1-AP

    IHC staining of mouse kidney using 55061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, BxPC-3 cells
Positive IHC detected inmouse kidney tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55061-1-AP targets XYLT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name xylosyltransferase I
Synonyms Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1
Calculated Molecular Weight 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberNM_022166
Gene Symbol XYLT1
Gene ID (NCBI) 64131
RRIDAB_10804758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y38
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.

实验方案

Product Specific Protocols
WB protocol for XYLT1 antibody 55061-1-APDownload protocol
IHC protocol for XYLT1 antibody 55061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Phytomedicine

Gua Lou Er Chen decoction attenuates atherosclerosis by reducing proteoglycans accumulation and inflammation

Authors - Hongya Guo
View Article