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验证数据展示

  • WB analysis of A431 using 26541-1-AP

    WB analysis of A431 using 26541-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 26541-1-AP (XYLB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 26541-1-AP

    IHC staining of human skin cancer using 26541-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin cancer using 26541-1-AP

    IHC staining of human skin cancer using 26541-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 40x lens).

XYLB Polyclonal antibody

XYLB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26541-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

XYLB, Xylulokinase, Xylulose kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26541-1-AP targets XYLB in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 XYLB fusion protein Ag19208 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 xylulokinase homolog (H. influenzae)
别名 XYLB, Xylulokinase, Xylulose kinase
计算分子量 536 aa, 58 kDa
观测分子量 43 kDa
GenBank蛋白编号BC137076
基因名称 XYLB
Gene ID (NCBI) 9942
RRIDAB_2880546
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75191
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human xylulokinase-like protein (XYLB), is a 58 kDa protein belongs to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. XYLB is a key regulator for controlling metabolic disease.XYLB also plays important roles in energy metabolism. XYLB has 2 isoforms produced by alternative splicing with the MW of 58 and 43 kDa.

实验方案

Product Specific Protocols
WB protocol for XYLB antibody 26541-1-APDownload protocol
IHC protocol for XYLB antibody 26541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols