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  • Featured Product
  • KD/KO Validated

XRN2 Polyclonal antibody

XRN2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA and More (5)

Conjugate

Unconjugated

Cat No : 11267-1-AP

Print datasheet

Synonyms

5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2

验证数据展示

  • WB analysis using 11267-1-AP

    WB analysis using 11267-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells, COLO 320 cells, HEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11267-1-AP targets XRN2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Cited ApplicationsWB, IP, IF, IHC, CoIP, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen XRN2 fusion protein Ag1788 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5'-3' exoribonuclease 2
Synonyms 5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC006417
Gene Symbol XRN2
Gene ID (NCBI) 22803
RRIDAB_2288787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

实验方案

Product Specific Protocols
WB protocol for XRN2 antibody 11267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
humanWB

Cell

Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.

Authors - Wagschal Alexandre A
  • KD Validated
View Article
humanChIP,CoIP,WB

EMBO J

Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids.

Authors - Sofiane Y Mersaoui
  • KD Validated
View Article

J Cell Biol

Intronless mRNAs transit through nuclear speckles to gain export competence.

Authors - Ke Wang
View Article
humanWB

PLoS Pathog

Characterization of miR-122-independent propagation of HCV.

Authors - Chikako Ono
  • KD Validated
View Article
humanWB, IF

J Virol

Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication.

Authors - You Li
  • KD Validated
View Article