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- KD/KO Validated
XRCC5/Ku80 Polyclonal antibody
XRCC5/Ku80 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, K-562 cells, HEK-293 cells, A431 cells, human liver tissue, HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16389-1-AP targets XRCC5/Ku80 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP, ChIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, pig |
Immunogen | XRCC5/Ku80 fusion protein Ag9454 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) |
Synonyms | 86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP 1, KARP1, Ku80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5, XRCC5/Ku80 |
Calculated Molecular Weight | 732 aa, 83 kDa |
Observed Molecular Weight | 80-83 kDa |
GenBank Accession Number | BC019027 |
Gene Symbol | XRCC5 |
Gene ID (NCBI) | 7520 |
RRID | AB_2257509 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13010 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for XRCC5/Ku80 antibody 16389-1-AP | Download protocol |
IHC protocol for XRCC5/Ku80 antibody 16389-1-AP | Download protocol |
IF protocol for XRCC5/Ku80 antibody 16389-1-AP | Download protocol |
IP protocol for XRCC5/Ku80 antibody 16389-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. | ||
Oncogene UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy. | ||
Phytomedicine Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer | ||
J Mater Chem B Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer | ||
FEBS J Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy | ||
Oncotarget Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages. |