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XRCC2 Polyclonal antibody

XRCC2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 20285-1-AP

Print datasheet

Synonyms

DNA repair protein XRCC2, XRCC2

验证数据展示

  • WB analysis of K-562 using 20285-1-AP

    WB analysis of K-562 using 20285-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20285-1-AP (XRCC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 20285-1-AP

    IP experiment of K-562 using 20285-1-AP

    IP result of anti-XRCC2 (IP:20285-1-AP, 4ug; Detection:20285-1-AP 1:500) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells, A375 cells, HEK-293 cells
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20285-1-AP is XRCC2 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen XRCC2 fusion protein Ag14011 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Synonyms DNA repair protein XRCC2, XRCC2
Calculated Molecular Weight 280 aa, 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2878662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for XRCC2 antibody 20285-1-APDownload protocol
IP protocol for XRCC2 antibody 20285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development

Authors - Nazareno Bona
View Article