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验证数据展示

  • WB analysis using 87251-1-RR (same clone as 87251-1-PBS)

    WB analysis using 87251-1-RR (same clone as 87251-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87251-1-RR (XRCC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87251-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 87251-1-RR (same clone as 87251-1-PBS)

    IF Staining of Jurkat using 87251-1-RR (same clone as 87251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using XRCC1 antibody (87251-1-RR, Clone: 252518B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87251-1-PBS in a different storage buffer formulation.

  • Affinity of 87251-1-RR

    Affinity of 87251-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87251-1-RR against Human XRCC1 were performed. The affinity constant is below 1 pM.

XRCC1 Recombinant monoclonal antibody, PBS Only

XRCC1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87251-1-PBS

产品说明书

CloneNo. 252518B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87251-1-PBS targets XRCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15172

Product name: Recombinant human XRCC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 284-633 aa of BC023593

Sequence: TAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 1
别名 DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
计算分子量 633 aa, 70 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC023593
基因名称 XRCC1
Gene ID (NCBI) 7515
RRIDAB_3745432
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18887
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.

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