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验证数据展示

  • WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    WB analysis using 66552-1-Ig (same clone as 66552-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    IHC staining of human testis using 66552-1-Ig (same clone as 66552-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66552-1-PBS in a different storage buffer formulation.

XLF Monoclonal antibody, PBS Only

XLF Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66552-1-PBS

产品说明书

CloneNo. 1F8C7

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66552-1-PBS targets XLF in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag2496

Product name: Recombinant human NHEJ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-299 aa of BC030986

Sequence: MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 nonhomologous end-joining factor 1
别名 NHEJ1, Protein cernunnos, XLF, XRCC4 like factor
计算分子量 35 kDa
观测分子量 37 kDa
GenBank蛋白编号BC030986
基因名称 XLF
Gene ID (NCBI) 79840
RRIDAB_2881914
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9Q4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

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