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验证数据展示

  • WB analysis of mouse pancreas using 12830-1-AP

    WB analysis of mouse pancreas using 12830-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12830-1-AP

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas cancer using 12830-1-AP

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 40x lens).

XK Polyclonal antibody

XK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12830-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse pancreas tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12830-1-AP targets XK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3550

Product name: Recombinant human XK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 371-444 aa of BC036019

Sequence: QFFHPCKKLFSSSVSEGFQRWLRCFCWACRQQKPCEPIGKEDLQSSRDRDETPSSSKTSPEPGQFLNAEDLCSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X-linked Kx blood group (McLeod syndrome)
别名 Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1
计算分子量 444 aa, 51 kDa
观测分子量 45-51 kDa
GenBank蛋白编号BC036019
基因名称 XK
Gene ID (NCBI) 7504
RRIDAB_2877887
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51811
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XK, also named as XKR1 and XRG1, is responsible for the Kx blood group system. XK forms a functional complex with the Kell glycoprotein. It represents a crucial factor for the maintenance of normal muscle structure and function.

实验方案

Product Specific Protocols
IHC protocol for XK antibody 12830-1-APDownload protocol
WB protocol for XK antibody 12830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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