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验证数据展示

  • WB analysis using 27057-1-AP

    WB analysis using 27057-1-AP

    Untreated and IFN alpha treated treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27057-1-AP (XAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 27057-1-AP

    IP experiment of Jurkat using 27057-1-AP

    IP result of anti-XAF1 (IP:27057-1-AP, 4ug; Detection:27057-1-AP 1:800) with Jurkat cells lysate 1920 ug.

  • WB analysis of Jurkat using 27057-1-AP

    WB analysis of Jurkat using 27057-1-AP

    Untreated and IFN alpha treated treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27057-1-AP (XAF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

XAF1 Polyclonal antibody

XAF1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27057-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

BIRC4 binding protein, BIRC4-binding protein, BIRC4BP, XIAP associated factor 1, XIAPAF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, IFN alpha treated treated Jurkat cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27057-1-AP targets XAF1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25843

Product name: Recombinant human XAF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 254-301 aa of BC073156

Sequence: PRGDKAAYDILRRCSQCGILLPLPILNQHQEKCRWLASSKGKQVRNFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 XIAP associated factor 1
别名 BIRC4 binding protein, BIRC4-binding protein, BIRC4BP, XIAP associated factor 1, XIAPAF1
计算分子量 301 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC073156
基因名称 XAF1
Gene ID (NCBI) 54739
RRIDAB_3742214
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6GPH4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XIAP-associated factor 1 (XAF1) is also named as BIRC4BP (BIRC4-binding protein) and XIAPAF1. XAF1 was originally identified as an antagonist of XIAP counteracting its anti-caspase activity by inhibiting the RING domain of XIAP. XAF1is also concerned with IFN-induced apoptosis (PMID: 35972291). XAF1 plays a key role in the communication between IRF1-mediated early immune responses and IRF3-mediated late IFN-dependent immune responses (PMID: 37595039). XAF1 as a direct interacting protein of AKT1, which strongly binds the N-terminal region of AKT1 to block its K63-linked poly-ubiquitination and subsequent activation (PMID: 37384528). XAF1 may serve as an osteoprotective regulator to restore bone homeostasis (PMID: 38957269).

实验方案

Product Specific Protocols
IP protocol for XAF1 antibody 27057-1-APDownload protocol
WB protocol for XAF1 antibody 27057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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