验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, IFN alpha treated treated Jurkat cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27057-1-AP targets XAF1 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag25843 Product name: Recombinant human XAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-301 aa of BC073156 Sequence: PRGDKAAYDILRRCSQCGILLPLPILNQHQEKCRWLASSKGKQVRNFS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | XIAP associated factor 1 |
| 别名 | BIRC4 binding protein, BIRC4-binding protein, BIRC4BP, XIAP associated factor 1, XIAPAF1 |
| 计算分子量 | 301 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC073156 |
| 基因名称 | XAF1 |
| Gene ID (NCBI) | 54739 |
| RRID | AB_3742214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6GPH4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XIAP-associated factor 1 (XAF1) is also named as BIRC4BP (BIRC4-binding protein) and XIAPAF1. XAF1 was originally identified as an antagonist of XIAP counteracting its anti-caspase activity by inhibiting the RING domain of XIAP. XAF1is also concerned with IFN-induced apoptosis (PMID: 35972291). XAF1 plays a key role in the communication between IRF1-mediated early immune responses and IRF3-mediated late IFN-dependent immune responses (PMID: 37595039). XAF1 as a direct interacting protein of AKT1, which strongly binds the N-terminal region of AKT1 to block its K63-linked poly-ubiquitination and subsequent activation (PMID: 37384528). XAF1 may serve as an osteoprotective regulator to restore bone homeostasis (PMID: 38957269).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for XAF1 antibody 27057-1-AP | Download protocol |
| WB protocol for XAF1 antibody 27057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
