验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, Y79 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | rat salivary gland tissue, mouse salivary gland tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10637-1-AP targets XAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | XAB2 fusion protein Ag0999 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | XPA binding protein 2 |
| 别名 | Pre-mRNA-splicing factor SYF1, PP3898, NTC90, KIAA1177, HCRN |
| 计算分子量 | 100 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC007208 |
| 基因名称 | XAB2 |
| Gene ID (NCBI) | 56949 |
| RRID | AB_2257336 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HCS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XAB2 antibody 10637-1-AP | Download protocol |
| IHC protocol for XAB2 antibody 10637-1-AP | Download protocol |
| IF protocol for XAB2 antibody 10637-1-AP | Download protocol |
| IP protocol for XAB2 antibody 10637-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.
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Nucleic Acids Res Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.
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Cell Death Dis XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.
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