验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25881-1-AP targets Whirlin in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Whirlin fusion protein Ag22807 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deafness, autosomal recessive 31 |
| 别名 | DFNB31, WHRN, USH2D, KIAA1526, CIP98 |
| 计算分子量 | 907 aa, 97 kDa |
| 观测分子量 | 46-50 kDa |
| GenBank蛋白编号 | BC014524 |
| 基因名称 | Whirlin |
| Gene ID (NCBI) | 25861 |
| RRID | AB_2880280 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P202 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Whirlin, also known as WHRN, DFNB31, and CIP98, is a cytoskeletal scaffold protein that plays an important role in the visual and auditory systems. In retina photoreceptors, Whirlin is required for the maintenance of periciliary membrane complexes that regulate intracellular protein transport. Mutations in Whirlin are associated with autosomal recessive nonsyndromic deafness and Usher syndrome(PMID: 17171570, 15841483).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Whirlin antibody 25881-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |